AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]

  • 货号:GF11030
AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]
  • AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]

Price: 询价

Size:
100 μg
Alternate Names
CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E
Clone No
Isotype
Armenian Hamster IgG
Leadtime
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Verified Samples Verified Samples in FCM: C57/BL6 Mouse splenocytes
Dilution
FCM 2 μg/mL(1×105-5×105 cells)
Background
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Immunogen Recombinant Mouse CD3ε protein
Abbre
CD3ε
Swissprot
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Purification Method
>98%, Protein A/G purified
Applications
FCM
Research Areas Immunology
Concentration
≥ 1 mg/mL
Conjugation
None (AF/LE)
Storage Buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Storage
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Expiration Date
12个月
Shipping
Ice bag
C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] (Right) and 0.2 μg Armenian Hamster IgG, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti- Armenian Hamster IgG Secondary Antibody, then anti-Mouse CD8a FITC-conjugated Monoclonal Antibody.
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