B7-2/CD86 Polyclonal Antibody

Verified Samples in WB: Romas
Verified Samples in IHC: Human tonsil

WB 1:500-1:1000, IHC 1:200-1:400

Polyclonal

IgG

Recombinant Rat B7-2/CD86 protein expressed by Mammalian

CD86

Activation B7-2 antigen, B72, B7-2, B-lymphocyte activation antigen B7-2, BU63, CD86 antigen, CD86 molecule, CD86, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86, CD28LG2B7-2 antigen), CD28 antigen ligand 2

37KDa

75kDa

Cell membrane, Secreted

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Antigen Affinity Purification

Unconjugated

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily.