beta Tubulin Monoclonal Antibody

货号：GA005310L

Price: 询价询价

Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: HT-29, Jurkat, 293T, Raw264.7, C6, Rat brain, Zebrafish Verified Samples in IF: U-2 OS
Dilution	WB 1:2000-1:4000, IF 1:200-1:400
Clonality	Monoclonal
Isotype	IgG
Immunogen	Recombinant human TUBB protein by E.coli
Abbre	beta Tubulin
Synonyms	UBB 1, TUBB 2, TUBB 5, TUBB, TUBB1, TUBB2
Swissprot
Calculated MW	55 kDa
Observed MW	55 kDa
Cellular Localization	Cytoplasm, cytoskeleton
Tissue Specificity	Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells
Clone No.	2E8-2
Conjugation	Unconjugated
Background	In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules,a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes,including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot.

Western blot with Anti beta Tubulin Monoclonal Antibody at dilution of 1:3000. Lane 1: HT-29 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: 293T cell lysate, Lane 4: Raw264.7 cell lysate, Lane 5: C6 cell lysate, Lane 6: Rat brain tissue lysate, Lane 7: Zebrafish tissue lysate.

Immunofluorescence analysis of U-2 OS cells using beta Tubulin Monoclonal Antibody at dilution of 1:200.

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武汉市高新大道858号光谷生物医药产业园二期B18栋