BRCA2 Polyclonal Antibody

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Hela, Rat testis, Jurkat, MCF-7, A431 Verified Samples in IHC: Human colon, Human breast Verified Samples in IF: U-2OS, U-251MG
Dilution	WB 1:1000-1:3000, IHC 1:100-1:200, IF 1:100-1:400
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Human Breast cancer type 2 susceptibility protein
Abbre	BRCA2
Synonyms	BRCC 2, BROVCA2, Breast and ovarian cancer susceptibility gene early onset antibody, FACD, FAD 1, FANCB, GLM3, BRCA 2
Swissprot
Calculated MW	384 kDa
Observed MW	384 kDa
Cellular Localization	Nucleus, Cytoskeleton.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Western Blot analysis of Jurkat, MCF-7 and A431 cells using BRCA2 Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Hela cells using BRCA2 Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat testis using BRCA2 Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunohistochemistry of paraffin-embedded Human breast using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of U-2OS cells using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of U-251MG cells using BRCA2 Polyclonal Antibody at dilution of 1:100

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武汉市高新大道858号光谷生物医药产业园二期B18栋