Elab Fluor® 647 Anti-Mouse TNFα Antibody[XT3.11]

货号：GFH00567M

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100 Tests 50 Tests 100 Tests × 2

Alternate Names	Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor, Differentiation inducing factor, TNFSF-2, TNF-a, TNF-alpha
Cellular Localization	Secreted
Clone No	XT3.11
Isotype	Rat IgG1, κ
Leadtime	咨询
Background	TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Abbre	TNF-α
Swissprot	P06804
Host	Rat
Reactivity	Mouse
Clonality	Monoclonal
Isotype Control	Elab Fluor® 647 Rat IgG1, κ Isotype Control[HRPN]
Applications	ICFCM
Form	Liquid
Concentration	5 μL/Test
Conjugation	Elab Fluor®647
Conjugation Information	Elab Fluor^® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration Date	12个月
Shipping	冰袋
Gene ID	21926

Mouse splenocytes were stimulated with Cell Stimulation MIX and Protein Transport Inhibitor MIX for 5 hours.Cells were stained with FITC Anti-Mouse CD4 Antibody and Elab Fluor^® 647 Rat IgG1,κ Isotype Control (left) or Elab Fluor^® 647 Anti-Mouse TNF-α[XT3.11] (right). Cells in the lymphocytes gate were used for analysis.

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