ENO1 Polyclonal Antibody

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Hela Verified Samples in IHC: Rat liver, Mouse brain
Dilution	WB 1:500-1:1000, IHC 1:50-1:100
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Human Alpha-enolase protein
Abbre	ENO1
Synonyms	2 phospho D glycerate hydro lyase, 2-phospho-D-glycerate hydro-lyase, Alpha enolase, Alpha enolase like 1, Alpha-enolase, C myc promoter binding protein, C-myc promoter-binding protein, EC 4.2.1.11, ENO1L1, ENOA, Enol, Enolase 1 (alpha), Enolase 1 (alpha) like 1, eno1
Swissprot
Calculated MW	47 kDa
Observed MW	47 kDa
Cellular Localization	Nucleus and Cytoplasm. Cell membrane. Cytoplasm>myofibril>sarcomere>M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction
Conjugation	Unconjugated
Background	Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.

Western Blot analysis of Hela cells using ENO1 Polyclonal Antibody at dilution of 1:600

Immunohistochemistry of paraffin-embedded Rat liver using ENO1 Polyclonal Antibody at dilution of 1:50

Immunohistochemistry of paraffin-embedded Mouse brain using ENO1 Polyclonal Antibody at dilution of 1:50

027-65384716

svipmaster@elabscience.cn

武汉市高新大道858号光谷生物医药产业园二期B18栋