FITC-conjugated Flag-Tag Monoclonal Antibody

  • 货号:GA00299C
FITC-conjugated Flag-Tag Monoclonal Antibody
  • FITC-conjugated Flag-Tag Monoclonal Antibody

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Size:
100 μL 25 μL
Verified Samples
Verified Samples in FCM: 293F cells were transfected with a tag expression plasmid to prepare an overexpression cell model
Dilution
FCM 0.2 μg per 106 cells in a 100 μL suspension
Clonality
Recombinant
Isotype
IgG2a
Immunogen
DYKDDDDK tag fusion protein
Abbre
Flag Tag
Synonyms
DDDDK epitope tag, DYKDDDDK epitope tag
Concentration
1 mg/mL
Storage
Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Buffer
PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4
Purification Method
Protein A/G Purification
Research Areas
Tags and Cell Markers, Microbiology, Immunology, Neuroscience
Clone No.
3H9
Conjugation
FITC
Amax
495nm
Emax
525nm
Background
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology,having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.
1X10^6 HEK-293F cells were intracellularly stained with 0.2 ug FITC-conjugated Flag-Tag Monoclonal Antibody (red), or 0.2 ug FITC-conjugated Flag-Tag Monoclonal Antibody Isotype Control (green).
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