FITC-conjugated Vinculin Monoclonal Antibody

货号：GA00297C

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in FCM: 293F cells were transfected with an expression plasmid to prepare an overexpression cell model
Dilution	FCM 0.2 μg per 10⁶ cells in a 100 μL suspension
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human Vinculin protein expressed by E.coli
Abbre	Vinculin
Synonyms	Metavinculin (MV), VCL, Vinculin
Swissprot
Cellular Localization	Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
Concentration	1 mg/mL
Storage	Store at 4°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Buffer	PBS with 0.05% proclin 300 and 0.1% gelatin,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Cardiovascular, Signal Transduction, Isotype/Loading Controls, Epigenetics and Nuclear Signaling
Clone No.	4D4
Conjugation	FITC
Amax	495nm
Emax	525nm
Background	Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific.

1X10^6 Hela cells were intracellularly stained with 0.2 ug FITC-conjugated Vinculin Monoclonal Antibody(red), or 0.2 ug FITC-conjugated Vinculin Monoclonal Antibody Isotype Control (green).

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