Flag-Tag Polyclonal Antibody
- 货号：GA405250L
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: Flag-Tagged fusion protein Verified Samples in IP: Hela, 293F |
| Dilution | WB 1:10000-1:20000, IP 3ug/sample, IF 1:2000-4000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Flag-Tag |
| Synonyms | DDDDK epitope tag |
| Calculated MW | 28 kDa |
| Observed MW | 28 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. |
Western blot analysis of 293F cells transfected with Flag tagged recombinant protein at different dilutions. lane 1: 1: 5000, lane 2: 1: 10000, lane 3: 1: 20000, lane 4: 1: 40000, lane 5: 1: 80000, lane 6: 1: 160000, lane 7: 1: 320000, lane 8: 1: 640000
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.
IP Result of 293F cells transfected with FLAG-Tag fusion protein, using anti-FLAG-Tag rabbit antibody. Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody
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