GAPDH Monoclonal Antibody
- 货号：GA005280L
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7, Hep G2, Raw264.7, Mouse brain, Rat lung, Rat brain, C6 Verified Samples in IHC: Human appendix |
| Dilution | WB 1:2000-1:4000, IHC 1:200-1:400 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Abbre | GAPDH |
| Synonyms | BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW | 36 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Metabolism, Cancer, Neuroscience, Isotype/Loading Controls, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers, Biochemicals, Cardiovascular, Developmental Biology |
| Clone No. | 7B8 |
| Conjugation | Unconjugated |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
Western blot with Anti GAPDH Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: MCF-7 cell lysate, Lane 3:Hep G2 cell lysate, Lane 4: Raw264.7 cell lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Rat lung tissue lysate, Lane 7: Rat brain lysate, Lane 8: C6 cell lysate.
Immunohistochemistry of paraffin-embedded Human breast cancer using GAPDH Monoclonal Antibody at dilution of 1:600.
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