GAPDH Polyclonal Antibody

Verified Samples in WB: A431, HepG2, Hela, HT29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart, Rat brain
Verified Samples in IHC: Human bladder cancer, Human kidney, Mouse colon, Rat colon

WB 1:3000-1:6000, IHC 1:300-1:500

Polyclonal

IgG

Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

GAPDH

BARS-38,  G3PDH,  GAPD,  KNC-NDS6,  OCAS,  cb609,  p38 component, aging associated gene 9 protein

35 kDa

35 kDa

Cytoplasm, Cytoskeleton, Nucleus.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Antigen Affinity Purification

Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism

Unconjugated

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.