GFP Monoclonal Antibody
- 货号：GA480100L
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Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: eGFP, eGFP protein with anti-eGFP Verified Samples in IF: zebrafish embryo Verified Samples in IP: 293 Verified Samples in FCM: CHO |
| Dilution | WB 1:100000-200000, IP 3μg/sample, IF 1:8000-16000, FCM 0.2μg |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant GFP protein expressed by E.coli |
| Abbre | GFP-Tag |
| Synonyms | GFP, Green fluorescent protein |
| Swissprot | |
| Calculated MW | 32 kDa |
| Observed MW | 32 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Protein A/G Purification |
| Clone No. | 5B5 |
| Conjugation | Unconjugated |
| Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.
Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.
IP Result of 293 cells transfected with GFP-Tag fusion protein, using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
1x10^6 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
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