GFP Monoclonal Antibody
- 货号：GA480430L
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: 293F cells transfected with the GFP-tag Verified Samples in IP: 293F cells transfected with the FLAG-GFP, 293F, anti-GFP-Tag monoclonal antibody |
| Dilution | WB 1:100000-200000, IF 1:8000-16000, IP 3ug/sample |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant GFP protein expressed by E.coli |
| Abbre | GFP-Tag |
| Synonyms | GFP, Green fluorescent protein |
| Swissprot | |
| Calculated MW | 32 kDa |
| Observed MW | 32 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 2A7 |
| Conjugation | Unconjugated |
| Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Western blot of GFP protein with anti-GFP at dilution of 1:50000, 1:100000, 200000. Lane 1:293F cells transfected with GFP-Tag fusion protein, lane 2: 293F cells transfected with GFP-Tag fusion protein, Lane 3:293F cells transfected with GFP-Tag fusion protein
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:50000.
IP Result of 293F cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
联系我们
027-65384716
svipmaster@elabscience.cn
武汉市高新大道858号光谷生物医药产业园二期B18栋
