GST-Tag Monoclonal Antibody

  • 货号:GA480060L
GST-Tag Monoclonal Antibody
  • GST-Tag Monoclonal Antibody

Price: 询价 询价

Size:
100 μL 25 μL
Verified Samples
Verified Samples in WB: four different GST-Tagged fused recombinant proteins
Dilution
WB 1:500-1000
Clonality
Monoclonal
Isotype
IgG
Immunogen
Recombinant GST protein expressed by E.coli
Abbre
GST-Tag
Synonyms
GST,  Glutathione S-transferase class-mu 26 kDa isozyme,  Sj26 antigen,  SjGST, Glutathione S Transferase
Calculated MW
26 kDa
Observed MW
26 kDa
Concentration
1 mg/mL
Storage
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer
PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method
Protein A/G Purification
Clone No.
4E7
Conjugation
Unconjugated
Background
GST , Glutathione-S-transferase, a 26 kDa sequence of 211 amino acids, is another widely used affinity tag that increases solubility of the desired protein. GST tag has affinity for immobilized glutathione and is used for prokaryotic expression more frequently. It can be fused to either the N-terminus or C-terminus of a protein.
Western blot of four different GST-Tagged fused recombinant proteins with Anti-GST rabbit monoclonal antibody at dilution of 1:1000. Lane 1 : Recombinant GST protein at 6.25ng; Lane 2 : Recombinant GST protein at 12.5ng; Lane 3: Recombinant GST protein at 25ng; Lane 4: Recombinant GST protein at 50ng
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