HA-Tag Monoclonal Antibody

货号：GA480210L

Price: 询价询价

Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: HA-Tag fused recombinant protein Verified Samples in IF: 293F Verified Samples in IP: 293F Verified Samples in FCM: CHO
Dilution	WB 1:5000-10000, IP 3μg/sample, IF 1:400-800, FCM 0.2μg
Clonality	Monoclonal
Isotype	IgG2a
Immunogen	Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Abbre	HA-Tag
Synonyms	HA1 , Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, hemagglutinin, HA epitope tag, HA2
Calculated MW	28 kDa
Observed MW	28 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS, pH 7.2. Contains 0.05% proclin 300.
Purification Method	Protein A/G Purification
Clone No.	5F7
Conjugation	Unconjugated
Background	The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

Western blotting with anti-HA-Tag monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate

Immunofluorescent analysis of 293F cells transfected with HA-Tag fusion protein using anti-HA-Tag monoclonal antibody at dilution of 1:800.

IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag monoclonal antibody .lane 1: mouse IgG2a Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag mouse monoclonal antibody

1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag mAb(E-AB-48021) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)

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