HA-Tag Monoclonal Antibody
- 货号：GA480220L
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: 293 Verified Samples in IF: 293F |
| Dilution | WB 1:5000-10000, IF 1:800-1600 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. |
| Abbre | HA-Tag |
| Synonyms | HA2, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, hemagglutinin, HA epitope tag, HA1 |
| Calculated MW | 28 kDa |
| Observed MW | 28 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 4F5 |
| Conjugation | Unconjugated |
| Background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Western blotting with anti-HA-Tag rabbit monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with the HA-GFP using anti-HA-Tag rabbit monoclonal Antibody at 1:2000 dilution.
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