HA-Tag Polyclonal Antibody
- 货号：GA405230L
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: lane1: HA tag transfected HEK 293 whole cell lysate, lane2: HEK 293whole cell lysate Verified Samples in IF: 293F Verified Samples in IP: 293F Verified Samples in FCM: 293F |
| Dilution | WB 1:2000-5000, IP 3μg/sample, IF 1:800-1600, FCM 0.2μg |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. |
| Abbre | HA-Tag |
| Synonyms | HA1, HA2, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, HA epitope tag |
| Calculated MW | 28 kDa |
| Observed MW | 28 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to int |
Western blotting with Anti-HA rabbit polyclonal antibody at dilution of 1:1000. Lane1: HA tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with the HA-GFP, using anti-HA-Tag Polyclonal Antibody at 1:800 dilution.
IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag rabbit polyclonal antibody. lane 1: rabbit IgG Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag rabbit antibody
1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag pAb(E-AB-40523) and AF647 conjugated Goat Anti-Rabbit IgG (H+L);lsotype Control stained with 0.2ug Rabbit IgG and AF647 conjugated Goat Anti-Rabbit IgG (H+L)
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