Histone H3 Polyclonal Antibody

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Mouse Spleen, HT-29, NIH/3T3, C6
Dilution	WB 1:5000-10000
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthetic peptide derived from N-terminus of human Histone H3 protein conjugated to keyhole limpet haemocyanin.
Abbre	Histone H3
Synonyms	H3/A, H31, H3FA, H3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Hist1h3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone cluster 1, histone 1, member A, H3 histone family
Swissprot
Calculated MW	15 kDa
Observed MW	15 kDa
Cellular Localization	Chromosome, Nucleosome core, Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. This antibody is not cross-reactive with other histones.

Western blot of Mouse spleen lysates with anti-Histone H3 pAb at dilution of 1:1000.

Western Blotting with anti-Histone H3 polyclonal antibody at dilution of 1:5000. Lane 1:HT-29 cell lysates, Lane 2: NIH/3T3 cell lysates, Lane 3: C6

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