HRP-conjugated beta actin Monoclonal Antibody

货号：GA00295HP

Price: 询价询价

Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Raji, Jurkat, HL-60, U87-MG, Mouse lung, Rat lung, Raw264.7, C6
Dilution	WB 1:2500-1:5000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human Beta actin protein by E. Coli
Abbre	beta actin
Synonyms	A26C1A, ACTB, Actin beta, Actin cytoplasmic 1, Actx, b actin
Swissprot
Calculated MW	43 kDa
Observed MW	43 kDa
Cellular Localization	Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Signal Transduction, Tags and Cell Markers, Isotype/Loading, Controls, Neuroscience, Biochemicals, Cancer, Cardiovascular, Cell Biology, Immunology, Metabolism, Epigenetics and Nuclear Signaling, Stem Cells
Clone No.	3A9
Conjugation	HRP
Background	Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR.

Western blot with HRP-conjugated beta actin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate,lane 2:Jurkat whole cell lysate, lane 3:HL-60 whole cell lysate,lane 4:U87-MG whole cell lysate,lane 5:mouse lung whole cell lysate,lane 6:rat lung whole cell lysate,lane 7:Raw264.7 whole cell lysate,lane 8:C6 whole cell lysate

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武汉市高新大道858号光谷生物医药产业园二期B18栋