HRP-conjugated Lamin A/C Monoclonal Antibody

Verified Samples in WB: HL-60, U87-MG, Hela, SH-SY5Y, THP-1

WB 1:2500-1:5000

Monoclonal

IgG1

Recombinant human Lamin A/C protein expressed by E.coli

Lamin A/C

LMN1, LMNA, Lamin A/C, Prelamin-A/C

65 kDa

65-70 kDa

Nucleus, Nucleus envelope, Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina, EMD is required for proper localization of non-farnesylated prelamin-A/C.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Protein A/G Purification

Signal Transduction, Tags and Cell Markers, Cancer, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Biochemicals

4F1

HRP

Lamin A/C is also named as LMNA,or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis,the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability,chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy,familial partial lipodystrophy,limb girdle muscular dystrophy,dilated cardiomyopathy,Charcot-Marie-Tooth disease,and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa,65 kDa,70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.