HRP-conjugated Vinculin Monoclonal Antibody

货号：GA00297HP

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Raji, HL-60, U87-MG, Mouse lung, Rat lung, C6
Dilution	WB 1:2500-1:5000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human Vinculin protein expressed by E.coli
Abbre	Vinculin
Synonyms	Metavinculin (MV), VCL, Vinculin
Swissprot
Calculated MW	124 kDa
Observed MW	135 kDa
Cellular Localization	Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Cardiovascular, Signal Transduction, Isotype/Loading Controls, Epigenetics and Nuclear Signaling
Clone No.	4D4
Conjugation	HRP
Background	Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific.

Western blot with HRP-conjugated Vinculin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate, lane 2:HL-60 whole cell lysate, lane 3:U87-MG whole cell lysate, lane 4:mouse lung whole cell lysate, lane 5:rat lung whole cell lysate, lane 6:C6 whole cell lysate

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