Lamin A/C Monoclonal Antibody

Verified Samples in WB: SH-SY5Y, HL-60, U-87 MG, Hep G2
Verified Samples in IF: HeLa, U-2 OS

WB 1:2000-1:4000, IF 1:500-1:1000

Monoclonal

IgG1

Recombinant human Lamin A/C protein expressed by E.coli

Lamin A/C

Prelamin-A/C, LMNA, LMN1, Lamin A/C

65 kDa

70,80 kDa

Nucleus lamina, Nucleus envelope, Nucleus

In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Protein A/G Purification

Signal Transduction, Tags and Cell Markers, Cancer, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Biochemicals

4F1

Unconjugated

Lamin A/C is also named as LMNA,or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis,the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability,chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy,familial partial lipodystrophy,limb girdle muscular dystrophy,dilated cardiomyopathy,Charcot-Marie-Tooth disease,and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa,65 kDa,70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.