NPM1 Polyclonal Antibody

Verified Samples in WB: Hela, 293T, A549, Jurkat, NIH/3T3, PC-3, Raji, Caco-2
Verified Samples in IHC: Mouse colon, Rat colon

WB 1:10000-1:20000, IHC 1:200-1:500

Polyclonal

IgG

Recombinant Mouse Nucleophosmin protein

NPM1

MGC104254,  NO38,  NPM,  NPM1,  Nucleolar phosphoprotein B23,  Nucleolar protein NO38,  Nucleophosmin,  Nucleophosmin (nucleolar phosphoprotein B23 numatrin),  Nucleophosmin/nucleoplasmin family member 1,  Num,  nucleophosmin nucleoplasmin family member 1, B23

32 kDa

37 kDa

Cytoplasm, Cytoskeleton, Nucleus.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Antigen Affinity Purification

Cancer, Epigenetics and Nuclear Signaling

Unconjugated

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective,multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates,dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.