PCNA Monoclonal Antibody

Verified Samples in WB: HT-29, Jurkat, THP-1, Mouse spleen, Raw264.7, C6, HeLa, NIH/3T3
Verified Samples in IP: THP-1
Verified Samples in IHC: Human lung cancer, Human ovary cancer

WB 1:2000-1:4000, IP 4ug/sample, IHC 1:200-1:400

Monoclonal

IgG2a

Recombinant human PCNA protein expressed by E.coli

PCNA

Proliferating cell nuclear antigen,PCNA,Cyclin

29 kDa

33 kDa

Nucleus 

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Protein A/G Purification

Cell Biology, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Cancer, Isotype, Loading Controls

9C6

Unconjugated

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.