PCNA Monoclonal Antibody

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Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Zebrafish Verified Samples in IHC: Zebrafish
Dilution	WB 1:1000-2000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human PCNA protein expressed by E.coli
Abbre	PCNA
Synonyms	Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNAR, Pcna/cyclin, Polymerase delta accessory protein, Proliferating ce, cb16, etID36690.10, fa28e03, fb36g03, ATLD2
Swissprot
Calculated MW	30 kDa
Observed MW	36 kDa
Cellular Localization	Nucleus
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Protein A/G Purification
Research Areas	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers
Clone No.	8B12
Conjugation	Unconjugated
Background	Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.

Western blot of Zebrafish whole lysates with anti-PCNA monoclonal antibody at dilution of 1:1000.

Immunofluorescence of Zebrafish frozen section with anti-PCNA monoclonal antibodyat dilution of 1:100.

Immunohistochemistry of Zebrafish paraffin embedded section with anti-PCNA mcab at dilution of 1:100.

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svipmaster@elabscience.cn

武汉市高新大道858号光谷生物医药产业园二期B18栋