PCNA Polyclonal Antibody

Price: 询价询价

Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: HepG2, Hela, RAW264.7, Raji, MCF-7 Verified Samples in IHC: Human colon cancer, Mouse testis, Rat testis
Dilution	WB 1:500-1:2000, IHC 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Human Proliferating cell nuclear antigen protein
Abbre	PCNA
Synonyms	Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, PCNAR, Pcna/cyclin, Polymerase delta accessory protein, cb16, etID36690.10, fa28e03, fb36g03, ATLD2
Swissprot
Calculated MW	28 kDa
Observed MW	34 lDa
Cellular Localization	Nucleus
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell markers, Cancer, Isotype/Loading Controls
Conjugation	Unconjugated
Background	This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Western Blot analysis of HepG2, Hela, Raw264.7 Raji and MCF-7 cells using PCNA Polyclonal Antibody at dilution of 1:500

Immunohistochemistry of paraffin-embedded Human colon cancer using PCNA Polycloanl Antibody at dilution of 1:50

Immunohistochemistry of paraffin-embedded Mouse testis using PCNA Polycloanl Antibody at dilution of 1:50

Immunohistochemistry of paraffin-embedded Rat testis using PCNA Polycloanl Antibody at dilution of 1:50

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武汉市高新大道858号光谷生物医药产业园二期B18栋