PCNA Polyclonal Antibody

Verified Samples in WB: Jurkat, A431, 293T

WB 1:3000-1:7000

Polyclonal

IgG

Synthetic peptide of Human Proliferating cell nuclear antigen

PCNA

Cyclin,  DNA polymerase delta auxiliary protein,  HGCN8729,  MGC8367,  Mutagen-sensitive 209 protein,  PCNA,  PCNAR,  Pcna/cyclin,  Polymerase delta accessory protein,  Proliferating cell nuclear antigen,  cb16,  etID36690.10,  fa28e03,  fb36g03, ATLD2

28 kDa

35 kDa

Nucleus

Highest expressed in oocyte

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Antigen Affinity Purification

Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers

Unconjugated

Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.