Propidium Iodide (PI) Solution(750 μM)

货号：GCQ0157

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Size:

500 Tests 100 Tests 500 Tests × 10

检测原理	PI是一种核酸染料，由于死细胞的细胞膜选择透过性丧失，碘化丙啶（Propidium Iodide，PI）可以进入胞内与双链DNA特异性结合并产生强烈的红色荧光 (Ex/Em=535nm/617nm)，从而对死细胞进行标记。
检测方法	荧光法;流式
样本类型	细胞
检测时间	30 min
检测仪器	流式细胞仪;荧光显微镜
荧光	PI
Ex/Em	535/617
Channel set	PE/PerCP/Cy5.5
Filter set	TRITC
自备试剂	PBS(E-BC-R187)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.

Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.

Jurkat cells were placed at 4 °C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.

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svipmaster@elabscience.cn

武汉市高新大道858号光谷生物医药产业园二期B18栋