Recombinant beta actin Monoclonal Antibody
- 货号：GA004840L
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- +7
Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: A431, Caco-2, Jurkat, K562, HeLa, PC-3, Ramos, TF-1, U20-S, HL-60, HT-29, Raji, HepG2, Raw264.7, C2C12, Mouse lung, Mouse pancreas, Mouse brain, Mouse breast, C6, PC-12, Rat spleen, Rat kidney, Rat lung, Rat pancreas, Rat brain, Rat stomach, Rat testis, Rat thymus, Rat breast Verified Samples in IHC: Human colon cancer, Human appendix, Human breast cancer, Human lung cancer, Rat ovary, Mouse ovary Verified Samples in IF: U-2 OS, NIH/3T3 |
| Dilution | WB 1:5000-1:20000, IHC 1:500-1:1000, IF 1:500-1:1000 |
| Clonality | Recombination |
| Isotype | IgG |
| Immunogen | Synthetic peptide within N-terminal residues of β-Actin |
| Abbre | beta actin |
| Synonyms | Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A, TUBA1A, TUBA3 |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Tags and Cell Markers, Isotype/Loading, Controls, Neuroscience, Biochemicals, Cancer, Cardiovascular, Cell Biology, Immunology, Metabolism, Epigenetics and Nuclear Signaling, Stem Cells |
| Clone No. | SA30-01 |
| Conjugation | Unconjugated |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR. |
Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. Lane 1.A431 cell lysate, Lane 2.Caco-2 cell lysate, Lane 3.Jurkat cell lysate, Lane 4.K562 cell lysate, Lane 5.HeLa cell lysate, Lane 6.PC-3 cell lysate, Lane 7.Ramos cell lysate, Lane 8.TF-1 cell lysate, Lane 9.U20-S cell lysate, Lane 10.HL-60 cell lysate, Lane 11.HT-29 cell lysate, Lane 12.Raji cell lysate, Lane 13.HepG2 cell lysate
Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. lane 1: Raw264.7 cell lysate, lane 2: C2C12 cell lysate, lane 3: mouse lung tissue lysate, lane 4: mouse pancreas tissue lysate, lane 5: mouse brain tissue lysate, lane 6: mouse breast tissue lysate
Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. lane 1: C6 cell lysate, lane 2: PC-12 cell lysate, lane 3: rat spleen tissue lysate, lane 4: rat kidney tissue lysate, lane 5: rat lung tissue lysate, lane 6: rat pancreas tissue lysate, lane 7: rat brain tissue lysate, lane 8: rat stomach tissue lysate, lane 9: rat testis tissue lysate, lane 10: rat thymus tissue lysate, lane 11: rat breast tissue lysate
Immunofluorescence analysis of U-2 OS cells using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000
Immunofluorescence analysis of NIH/3T3 cells using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded rat ovary using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human appendix using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human breast cancer using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.
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