Recombinant GAPDH Monoclonal Antibody
- 货号：GA004430L
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Price:
Size:
100 μL
25 μL
| Verified Samples |
Verified Samples in WB: A431, Caco-2, Jurkat, K562, HeLa, PC-3, Ramos, TF-1, U20-S, HL-60, HT-29, Raji, HepG2, Mouse heart, Mouse liver, Mouse uterus, Mouse lung, Mouse kidney, Mouse brain, Mouse stomach, C6, PC-12, Rat heart, Rat liver, Rat spleen, Rat brain, Rat stomach, Rat testis, Rat thymus, Rat skeletal muscle Verified Samples in IHC: Human colon cancer, Human appendix, Human breast cancer, Human lung cancer, Rat ovary, Mouse ovary Verified Samples in IF: U-2 OS cells, NIH/3T3 cells, C6 |
| Dilution | WB 1:20000-1:100000, IHC 1:2000-1:4000, IF 1:2000-1:4000 |
| Clonality | Recombination |
| Isotype | IgG |
| Immunogen | Recombinant protein within Mouse GAPDH |
| Abbre | GAPDH |
| Synonyms | BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW | 36 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Isotype, Loading Controls, Signal Transduction, Cancer, Metabolism |
| Clone No. | 20B8 |
| Conjugation | Unconjugated |
| Background | GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: A431 cell lysate,Lane 2: Caco-2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: K562 cell lysate, Lane 5: HeLa cell lysate, Lane 6: PC-3 cell lysate, Lane 7: Ramos cell lysate, Lane 8: TF-1 cell lysate, Lane 9: U20-S cell lysate, Lane 10: HL-60 cell lysate, Lane 11: HT-29 cell lysate, Lane 12: Raji cell lysate, Lane 13: HepG2 cell lysate
Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate
Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: C6 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat liver tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8: Rat testis tissue lysate, Lane 9: Rat thymus tissue lysate, Lane 10: Rat skeletal muscle tissue lysate
Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunofluorescence analysis of U-2 OS cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000
Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000
Immunofluorescence analysis of C6 using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human appendix using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded human breast cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
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