Recombinant Human CD274 Protein(His Tag)

B7 H;B7 H1;B7 homolog 1;B7-H1;B7H;B7H1;CD 274;CD274;CD274 antigen;CD274 molecule;MGC142294;MGC142296;OTTHUMP00000021029;PD L1;PD-L1;PD1L1;PDCD1 ligand 1;PDCD1L1;PDCD1LG1;PDL 1;PDL1;Programmed cell death 1 ligand 1;Programmed death ligand 1;RGD1566211

Mammalian

Met1-Thr239

Q9NZQ7

Human

26.1 kDa

35-40 kDa

C-His

Not validated for activity

> 90% as determined by reducing SDS-PAGE.

< 1.0 EU/mg of the protein as determined by the LAL method

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

This product is provided as lyophilized powder which is shipped with ice packs.

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Programmed cell death ligand 1(CD274,or B7-H1,PD-L1),is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with an their CD28 family receptors,the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell,and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal,essential for T-cell proliferation and production of IL10 and IFNG,in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with an a calculated molecular weight of 33 kDa,the apparent molecular weight has been reported as 45-70 kDa,suggesting probable glycosylation .