Strep-Tag II Monoclonal Antibody

Verified Samples in WB: Strep Ⅱ-Tag fused recombinant protein

WB 1:1000-2000

Monoclonal

IgG1

Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin.

Strep-Tag II

Strep II, Strep Tag II

45 kDa

45 kDa

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4

Protein A/G Purification

4H10

Unconjugated

The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.