25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit
中文名称：25羟基维生素D3(25-HVD3)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用25-HVD3抗原包被于酶标板上，实验时样品或标准品中的25-HVD3与包被的25-HVD3竞争生物素标记的抗25-HVD3单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，25-HVD3浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中25-HVD3的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的25-HVD3，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Maternal Vitamin D-3 Supplementation in an Oxidized-Oil Diet Protects Fetus from Developmental Impairment and Ameliorates Oxidative Stress in Mouse Placenta and Fetus
IF: 3.700Journal： JOURNAL OF NUTRITION (2024)Reactivity：MouseSample Type：serum,fetus -
Effects of Klotho protein, vitamin D, and oxidative stress parameters on urinary stone formation and recurrence
IF: 2.000Journal： INTERNATIONAL UROLOGY AND NEPHROLOGY (2024)PMID：38194188Reactivity：HumanSample Type：serum -
Mahanimbine Attenuates Ovariectomy-Induced Osteoporosis in Rats by Regulating Bone Turnover Markers and SIRT1-NF-κB Pathway
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：RatSample Type：serum -
Vitamin D improves hepatic steatosis in NAFLD via regulation of fatty acid uptake and β-oxidation
IF: 5.200 -
Vitamin D3 supplementation shapes the composition of gut microbiota and improves some obesity parameters induced by high-fat diet in mice
IF: 5.000 -
Effect of vitamin D3 on lipid droplet growth in adipocytes of mice with HFD-induced obesity
IF: 3.900 -
Unraveling the interaction between lead and calcium in occupationally exposed males: an exploratory observation study
IF: 3.000Journal： INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH (2023)PMID：37889332Reactivity：HumanSample Type：Serum -
Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats
IF: 6.100 -
Lactobacillus rhamnosus GG Promotes Intestinal Vitamin D Absorption by Upregulating Vitamin D Transporters in Senile Osteoporosis
IF: 4.000 -
Chronic vitamin D3 supplementation alleviates cognition impairment via inhibition of oxidative stress regulated by PI3K/AKT/Nrf2 in APP/PS1 transgenic mice
IF: 3.197
实验操作视频
