4-HNE(4-Hydroxynonenal) ELISA Kit
中文名称：4-羟基壬烯酸(4-HNE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.63-40 ng/mL
- 灵敏度： 0.38 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用4-HNE抗原包被于酶标板上，实验时样品或标准品中的4-HNE与包被的4-HNE竞争生物素标记的抗4-HNE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，4-HNE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中4-HNE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.63-40 ng/mL |
| 灵敏度 | 0.38 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的4-HNE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Unlocking Synergistic Hepatoprotection: Dapagliflozin and Silymarin Combination Therapy Modulates Nuclear Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway in Carbon Tetrachloride-Induced Hepatotoxicity in Wistar Rats
IF: 3.600 -
Knockout of TNF-α in microglia decreases ferroptosis and convert microglia phenotype after spinal cord injury
IF: 3.400Journal： Heliyon (2024)Reactivity：MouseSample Type：spinal cord tissue -
Determination of oxidative stress and copeptin levels of COVID-19 according to the clinical course
IF: 3.300Journal： MICROBIAL PATHOGENESIS (2024)Reactivity：HumanSample Type：Serum -
Nicotinamide mononucleotide mitigates neuroinflammation by enhancing GPX4-mediated ferroptosis defense in microglia
IF: 2.700Journal： BRAIN RESEARCH (2024)Reactivity：MouseSample Type：brain tissue -
Modelling ferroptosis in IPEC-J2 cells: insights into iron-dependent cell death
IF: 2.200 -
miR-1268a Regulates Fatty Acid Metabolism by Targeting CD36 in Angiotensin II-induced Heart Failure
IF: 1.800 -
A common East-Asian ALDH2 mutation causes metabolic disorders and the therapeutic effect of ALDH2 activators
IF: 16.600 -
Cholesterol confers ferroptosis resistance onto myeloid-biased hematopoietic stem cells and prevents irradiation-induced myelosuppression
IF: 11.400 -
Reductive stress induced by NRF2/G6PD through glucose metabolic reprogramming promotes malignant transformation in Arsenite-exposed human keratinocytes
IF: 9.800 -
Stroke by inducing HDAC9-dependent deacetylation of HIF-1 and Sp1, promotes TfR1 transcription and GPX4 reduction, thus determining ferroptotic neuronal death
IF: 9.200Journal： International Journal of Biological Sciences (2023)PMID：37324938Reactivity：Rat,Human,MouseSample Type：cortex
实验操作视频
