4-HNE(4-Hydroxynonenal) ELISA Kit
中文名称：4-羟基壬烯酸(4-HNE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.63-40 ng/mL
- 灵敏度： 0.38 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用4-HNE抗原包被于酶标板上，实验时样品或标准品中的4-HNE与包被的4-HNE竞争生物素标记的抗4-HNE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，4-HNE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中4-HNE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.63-40 ng/mL |
| 灵敏度 | 0.38 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的4-HNE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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实验操作视频
