8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Ursolic Acid Protects Neurons in Temporal Lobe Epilepsy and Cognitive Impairment by Repressing Inflammation and Oxidation
IF: 5.988Journal： Frontiers in Pharmacology (2022)PMID：35677445Reactivity：RatSample Type：hippocampal tissue -
Suppression of glutathione system and upregulation of caspase 3-dependent apoptosis mediate rohypnol-induced gastric injury
IF: 5.696 -
Atorvastatin-mediated downregulation of VCAM-1 and XO/UA/caspase 3 signaling averts oxidative damage and apoptosis induced by ovarian ischaemia/reperfusion injury
IF: 5.696 -
A green-inspired method to prepare non-split high-density lipoprotein (HDL) carrier with anti-dysfunctional activities superior to reconstituted HDL
IF: 5.589Journal： EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2022)PMID：36529255Reactivity：Mouse -
Spiromesifen conferred abnormal development in zebrafish embryos by inducing embryonic cytotoxicity via causing oxidative stress
IF: 5.202 -
Xiehuo Xiaoying decoction inhibits Tfh cell expansion and promotes Tfr cell amplification to ameliorate Graves’ disease
IF: 5.195 -
The involvement of oxidative stress, neuronal lesions, neurotransmission impairment, and neuroinflammation in acrylamide-induced neurotoxicity in C57/BL6 mice
IF: 5.190Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022)PMID：35088269Reactivity：MouseSample Type：brain -
3-Indolepropionic acid prevented chlorpyrifos-induced hepatorenal toxicities in rats by improving anti-inflammatory, antioxidant, and pro-apoptotic responses and abating DNA damage
IF: 5.190Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022)PMID：35644820Reactivity：RatSample Type：Hepatic , renal -
Zinc normalizes hepatic lipid handling via modulation of ADA/XO/UA pathway and caspase 3 signaling in highly active antiretroviral therapy-treated Wistar rats
IF: 5.168Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2022)Reactivity：RatSample Type：plasma,hepatic tissues -
Possible mechanisms involved in the testicular-protective property of quercetin in rats exposed to endosulfan toxicity
IF: 4.966Journal： PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2022)PMID：36464344Reactivity：RatSample Type：testicular tissue
实验操作视频
