8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒

货号：E-EL-0028
文献（222）
说明书

说明书

别称：

7,8-Dihydro-8-oxo-2'-deoxyguanosine、7,8-Dihydro-8-oxodeoxyguanosine、8-Hydroxy-2'-deoxyguanosine、8-Hydroxydeoxyguanosine、8-OH-dG、8-Oxo-2'-deoxyguanosine、8-Oxo-7,8-dihydro-2'-deoxyguanosine、8-Oxo-7,8-dihydrodeoxyguanosine、8-Oxo-dG

8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Universal
检测范围： 1.56-100 ng/mL
灵敏度： 0.94 ng/mL

产品应用	ELISA
检测原理	本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。
反应类型	Competitive-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	2 h 30 min
反应性	Universal
检测方法	比色法;ELISA;竞争法
检测范围	1.56-100 ng/mL
灵敏度	0.94 ng/mL
样本体积	50 μL
样本类型	血清、血浆等其他生物体液
特异性	可检测样本中的8-OHdG，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Perturbations in indices of oxidative stress, oxidative DNA damage and lung function in chronic exposure to wood dust in Southern Nigeria

IF: 2.1

Journal： INHALATION TOXICOLOGY (2023)

DOI：10.1080/08958378.2023.2224388

PMID：37326599

Reactivity：Human

Sample Type：serum
Heavy metals and inflammatory, oxidative/antioxidant and DNA damage biomarkers among people living with HIV/AIDS (PLWHA) in Niger Delta, Nigeria

IF: 2.100

Journal： JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING (2023)

DOI：10.1080/10934529.2023.2185004

Reactivity：Human

Sample Type：serum
Can follicular fluid 8-oxo-2′-deoxyguanosine predict the clinical outcomes in ICSI cycle among couples with normospermia male?

IF: 1.9

Journal： Obstetrics & Gynecology Science (2023)

DOI：10.5468/ogs.22170

PMID：37473783

Reactivity：Human

Sample Type：follicular fluid (FF)
Effects of autophagy inhibitor 3-methyladenine on a diabetic mice model

IF: 1.4

Journal： International Journal of Ophthalmology (2023)

DOI：10.18240/ijo.2023.09.12

PMID：37724274

Reactivity：Mouse

Sample Type：retinal tissues
Oxidative stress mediates hippocampal neuronal apoptosis through ROS/JNK/P53 pathway in rats with PTSD triggered by high-voltage electrical burn

IF: 1.2

Journal： FOLIA MORPHOLOGICA (2023)

DOI：10.5603/fm.95727

PMID：37691512

Reactivity：Rat，Human

Sample Type：serum
In vivo exposure to bisphenol F induces oxidative testicular toxicity: role of Erβ and p53/Bcl-2 signaling pathway

Journal： Frontiers in Reproductive Health (2023)

DOI：10.3389/frph.2023.1204728

Reactivity：Rat

Sample Type：testicular tissue
Ginsenoside Rd ameliorates high glucose-induced retinal endothelial injury through AMPK-STRT1 interdependence

IF: 10.334

Journal： PHARMACOLOGICAL RESEARCH (2022)

DOI：10.1016/j.phrs.2022.106123

PMID：35150861

Reactivity：Human
Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease

IF: 9.685

Journal： Cell Death & Disease (2022)

DOI：10.1038/s41419-022-05120-0

PMID：35908070

Reactivity：Mouse

Sample Type：Urine
Formation of Blood Neutrophil Extracellular Traps Increases the Mastitis Risk of Dairy Cows During the Transition Period

IF: 8.786

Journal： Frontiers in Immunology (2022)

DOI：10.3389/fimmu.2022.880578

PMID：35572521

Reactivity：Bovine

Sample Type：serum
New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway

IF: 8.101

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2022)

DOI：10.1016/j.freeradbiomed.2022.06.009

PMID：35697291

Reactivity：Human

Species

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Universal

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