8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Nephrotoxicity of nickel nano and microparticles in rat- a comparative, time dependent study with special reference to antioxidant defence system
IF: 1.514Journal： Inorganic and Nano-Metal Chemistry (2022)Reactivity：RatSample Type：urine -
Nephroprotective effects of polyherbal extract via attenuation of the severity of kidney dysfunction and oxidative damage in the diabetic experimental model
IF: 1.206Journal： CELLULAR AND MOLECULAR BIOLOGY (2022)Reactivity：RatSample Type：Urine -
Follicular fluid 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as biomarker for oxidative stress in intracytoplasmic sperm injection
Journal： JOURNAL OF MEDICAL INVESTIGATION (2022)PMID：35466131Reactivity：HumanSample Type：Follicular fluid (FF) -
Biomarkers of nucleic acid oxidation – A summary state-of-the-art
IF: 11.799 -
Association of Obstructive Sleep Apnea with the Aging Process
IF: 6.831Journal： Annals of the American Thoracic Society (2021)PMID：33662230Reactivity：HumanSample Type：blood -
Changes in genotoxicity, inflammatory and oxidative stress parameters of workers in marble processing plants
IF: 6.498 -
Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise
IF: 6.313 -
Fucoidan Protects against Acute Sulfoxaflor-Induced Hematological/Biochemical Alterations and Oxidative Stress in Male Mice
IF: 5.863 -
Indole-3-propionic acid mitigates chlorpyrifos-mediated neurotoxicity by modulating cholinergic and redox-regulatory systems, inflammatory stress, apoptotic responses and DNA damage in rats
IF: 4.860Journal： ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021)PMID：34915193Reactivity：RatSample Type：brain -
The Protective Effect of Heme Oxygenase-1 on Liver Injury Caused by DON-Induced Oxidative Stress and Cytotoxicity
IF: 4.546
实验操作视频
