8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Ameliorative Potential of Hydroethanolic Leaf Extract of Parquetina nigrescens on d-Galactose-Induced Testicular Injury
IF: 4.412 -
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study
IF: 4.380 -
Preservation of mitochondrial homeostasis is responsible for the ameliorative effects of Suhuang antitussive capsule on non-resolving inflammation via inhibition of NF-κB signaling and NLRP3 inflammasome activation
IF: 4.360Journal： JOURNAL OF ETHNOPHARMACOLOGY (2021)PMID：33460751Reactivity：HumanSample Type：Cell culture supernatant(THP-1 cells) -
Cardiovascular Risk Factors and Their Relationship with Vascular Dysfunction in South African Children of African Ancestry
IF: 4.242 -
Protective Effect of Chitosan Oligosaccharide against Hydrogen Peroxide-Mediated Oxidative Damage and Cell Apoptosis via Activating Nrf2/ARE Signaling Pathway
IF: 3.911 -
Ecotoxicological Effects of Bimetallic PdNi/MWCNT and PdCu/MWCNT Nanoparticles onto DNA Damage and Oxidative Stress in Earthworms
IF: 3.738 -
The Protective Role of E-64d in Hippocampal Excitotoxic Neuronal Injury Induced by Glutamate in HT22 Hippocampal Neuronal Cells
IF: 3.599 -
Potential contribution of ELISA and LFI assays to assessment of the oxidative stress condition based on 8-oxodG biomarker
IF: 3.365Journal： ANALYTICAL BIOCHEMISTRY (2021)PMID：33957135Reactivity：HumanSample Type：Serum,Plasma,Saliva,Urine,Aqueous Homor,Tissue homogenate -
Bromelain protects against cisplatin-induced ocular toxicity through mitigating oxidative stress and inflammation
IF: 3.356Journal： DRUG AND CHEMICAL TOXICOLOGY (2021)Reactivity：RatSample Type：ocular tissue -
Effects of aerobic exercise on lipopolysaccharide-induced experimental acute lung injury in the animal model of type 1 diabetes mellitus
IF: 2.969
实验操作视频
