8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Baicalein-ameliorated cerebral ischemia-reperfusion injury dependent on calpain 1/AIF pathway
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The effect of hypothermia in a rat testicular torsion/detorsion model
IF: 1.830Journal： Journal of Pediatric Urology (2021)PMID：33610458Reactivity：RatSample Type：Serum,Tissue homogenate(testis) -
A preliminary study on household air pollution exposure and health-related factors among rural housewives in Gansu province, northwest China
IF: 1.663Journal： Archives of Environmental & Occupational Health (2021)PMID：34748471Reactivity：HumanSample Type：urine -
High serum 8-hydroxy-2′-deoxyguanosine levels predict DNA damage and aging in professional divers
IF: 1.209Journal： Revista da Associacao Medica Brasileira (2021)PMID：34909901Reactivity：HumanSample Type：serum -
Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study
Journal： International Journal of Reproductive Biomedicine (2021)PMID：33718758Reactivity：HumanSample Type：Semen sample -
Apoptotic inducement of neuronal cells by codeine: possible role of disrupted redox state and caspase 3 signaling
-
DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25
IF: 42.779 -
Effect of acute exposure of saxitoxin on development of zebrafish embryos (Danio rerio)
IF: 5.715Journal： ENVIRONMENTAL RESEARCH (2020)PMID：32247151Reactivity：ZebrafishSample Type：Tissue homogenate -
Winter vacation, indoor air pollution and respiratory health among rural college students: A case study in Gansu Province, China
IF: 4.971Journal： BUILDING AND ENVIRONMENT (2020)Reactivity：HumanSample Type：Urine
实验操作视频
