8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Nodularin induced oxidative stress contributes to developmental toxicity in zebrafish embryos
IF: 4.872Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)PMID：32169726Reactivity：ZebrafishSample Type：Tissue homogenate -
Dibutyl phthalate aggravated asthma-like symptoms through oxidative stress and increasing calcitonin gene-related peptide release
IF: 4.872Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)PMID：32446102Reactivity：MouseSample Type：Tissue homogenate -
Berberine promotes peri-implant osteogenesis in diabetic rats by ROS-mediated IRS-1 pathway
IF: 4.734 -
Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice
IF: 4.018 -
Codeine exerts cardiorenal injury via upregulation of adenine deaminase/xanthine oxidase and caspase 3 signaling
IF: 3.647Journal： LIFE SCIENCES (2020)PMID：33159958Reactivity：RabbitSample Type：Serum,Plasma,Tissue homogenate -
Blood lead and cadmium levels in occupationally exposed workers and their effect on markers of DNA damage and repair
IF: 3.472Journal： ENVIRONMENTAL GEOCHEMISTRY AND HEALTH (2020)PMID：32813162Reactivity：HumanSample Type：Plasma -
Possible differences in the mechanism of malignant transformation of HaCaT cells by arsenite and its dimethyl metabolites, particularly dimethylthioarsenics
IF: 3.245Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2020)PMID：32416464Reactivity：HumanSample Type：Cell culture supernatant -
Polygonum minus essential oil modulates cisplatin-induced hepatotoxicity through inflammatory and apoptotic pathways
IF: 2.837 -
Codeine-induced sperm DNA damage is mediated predominantly by oxidative stress rather than apoptosis
IF: 2.753 -
Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO/TNF-α and caspase 3 activities
IF: 2.740
实验操作视频
