8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Glutathione-related antioxidant defence, DNA damage, and DNA repair in patients suffering from post-COVID conditions
IF: 2.700 -
The effects of human amnion membrane-derived mesenchymal stem cells conditioned medium on ionizing radiation-induced premature ovarian failure and endoplasmic reticulum stress-related apoptosis mechanism
IF: 2.600Journal： European Journal of Obstetrics & Gynecology and Reproductive Biology (2023)PMID：37566962Reactivity：RatSample Type：serum -
Transient receptor potential channel TRPM4 favors oxidized low-density lipoprotein-induced coronary endothelial cell dysfunction via a mechanism involving ferroptosis
IF: 2.600 -
Naringenin Prevents Renal Injury in Experimental Hyperuricemia Through Suppressing Xanthine Oxidase, Inflammation, Apoptotic Pathway, DNA Damage, and Activating Antioxidant System
IF: 2.100Journal： Metabolic Syndrome and Related Disorders (2023)PMID：37195720Reactivity：RatSample Type：kidney -
Perturbations in indices of oxidative stress, oxidative DNA damage and lung function in chronic exposure to wood dust in Southern Nigeria
IF: 2.100 -
Can follicular fluid 8-oxo-2′-deoxyguanosine predict the clinical outcomes in ICSI cycle among couples with normospermia male?
IF: 1.900Journal： Obstetrics & Gynecology Science (2023)PMID：37473783Reactivity：HumanSample Type：follicular fluid (FF) -
Effects of autophagy inhibitor 3-methyladenine on a diabetic mice model
IF: 1.400Journal： International Journal of Ophthalmology (2023)PMID：37724274Reactivity：MouseSample Type：retinal tissues -
Oxidative stress mediates hippocampal neuronal apoptosis through ROS/JNK/P53 pathway in rats with PTSD triggered by high-voltage electrical burn
IF: 1.200Journal： FOLIA MORPHOLOGICA (2023)DOI：10.5603/fm.95727PMID：37691512Reactivity：Rat，HumanSample Type：serum -
Ginsenoside Rd ameliorates high glucose-induced retinal endothelial injury through AMPK-STRT1 interdependence
IF: 10.334 -
Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease
IF: 9.685
实验操作视频
