8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
中文名称：8羟基脱氧鸟苷(8-OHdG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用8-OHdG抗原包被于酶标板上，实验时样品或标准品中的8-OHdG与包被的8-OHdG竞争生物素标记的抗8-OHdG单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，8-OHdG浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中8-OHdG的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆等其他生物体液 |
| 特异性 | 可检测样本中的8-OHdG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Formation of Blood Neutrophil Extracellular Traps Increases the Mastitis Risk of Dairy Cows During the Transition Period
IF: 8.786 -
New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway
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Melatonin Prevents NaAsO2-Induced Developmental Cardiotoxicity in Zebrafish through Regulating Oxidative Stress and Apoptosis
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Biological Effects of Hydrogen Water on Subjects with NAFLD: A Randomized, Placebo-Controlled Trial
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Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma
IF: 7.675 -
Role of Nox4 in High Calcium-Induced Renal Oxidative Stress Damage and Crystal Deposition
IF: 7.468 -
Ambient ozone, and urban PM2.5 co-exposure, aggravate allergic asthma via transient receptor potential vanilloid 1-mediated neurogenic inflammation
IF: 7.129Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)PMID：35994908Reactivity：MouseSample Type：lung -
STING up-regulates VEGF expression in oxidative stress-induced senescence of retinal pigment epithelium via NF-κB/HIF-1α pathway
IF: 6.780 -
The Polymorphisms of Genes Encoding Catalytic Antioxidant Proteins Modulate the Susceptibility and Progression of Testicular Germ Cell Tumor
IF: 6.575 -
Blood neutrophil extracellular traps: a novel target for the assessment of mammary health in transition dairy cows
IF: 6.175Journal： Journal of Animal Science and Biotechnology (2022)PMID：36380371Reactivity：BovineSample Type：serum
实验操作视频
