AA(Arachidonic Acid) ELISA Kit
中文名称：花生四烯酸(AA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用AA抗原包被于酶标板上，实验时样品或标准品中的AA与包被的AA竞争生物素标记的抗AA单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，AA浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中AA的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的AA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Leptin-mediated suppression of lipoprotein lipase cleavage enhances lipid uptake and facilitates lymph node metastasis in gastric cancer
IF: 20.100Journal： Cancer Communications (2024)Reactivity：Human,MouseSample Type：MKN45 cell-Xenograft -
Targeted Liposomes Sensitize Plastic Melanoma to Ferroptosis via Senescence Induction and Coenzyme Depletion
IF: 17.100 -
MFSD7C protects hemolysis-induced lung impairments by inhibiting ferroptosis
IF: 14.700Journal： Nature Communications (2024)Reactivity：Human,MouseSample Type：lung tissue -
Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway
IF: 6.700Journal： PHYTOMEDICINE (2024)Reactivity：MouseSample Type：cortex tissue -
Inhibiting arachidonic acid generation mitigates aging-induced hyperinsulinemia and insulin resistance in mice
IF: 6.600Journal： CLINICAL NUTRITION (2024)Reactivity：MouseSample Type：serum -
Blastocyst-Derived Lactic Acid May Regulate S100A6 Expression and Function in Mouse Decidualization via Stimulation of Uterine Epithelial Arachidonic Acid Secretion
IF: 6.000 -
Serum Levels of Arachidonic Acid, Interleukin-6, and C-Reactive Protein as Potential Indicators of Pulmonary Viral Infections: Comparative Analysis of Influenza A, Respiratory Syncytial Virus Infection, and COVID-19
IF: 3.800 -
Shenqi Fuzheng injection modulates tumor fatty acid metabolism to downregulate MDSCs infiltration, enhancing PD-L1 antibody inhibition of intracranial growth in Melanoma
IF: 7.900Journal： PHYTOMEDICINE (2023)Reactivity：MouseSample Type：B16 brain transplant tumor -
Embryo-derive TNF promotes decidualization via fibroblast activation
IF: 7.700 -
COX-2/PGE2 upregulation contributes to the chromosome 17p-deleted lymphoma
IF: 6.200
实验操作视频
