ACH(Acetylcholine) ELISA Kit
中文名称：乙酰胆碱(ACH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ACH抗原包被于酶标板上，实验时样品或标准品中的ACH与包被的ACH竞争生物素标记的抗ACH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ACH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ACH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ACH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Human PERIOD3 variants lead to winter depression-like behaviours via glucocorticoid signalling
IF: 18.900Journal： Nature Metabolism (2024)Reactivity：MouseSample Type：adipose tissue -
The effect of combining an inhaled corticosteroid and a long-acting muscarinic antagonist on human airway epithelial cells in vitro
IF: 5.800 -
SIRT-2 inhibition by AK-7 orchestrates fibrotic cascades in airways through neuroimmune interaction via TRPA1, TRPM8 and TGF-β signalling
IF: 5.300Journal： BIOCHEMICAL PHARMACOLOGY (2024)Reactivity：MouseSample Type：Bronchoalveolar lavage fluid (BALF) -
Synergistic effects of Ligilactobacillus salivarius Li01 and psyllium husk prevent mice from developing loperamide-induced constipation
IF: 5.100 -
Resveratrol-Loaded Pluronic Micelles Ameliorate Scopolamine-Induced Cognitive Dysfunction Targeting Acetylcholinesterase Activity and Programmed Cell Death
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：cortical tissue,hippocampal tissue -
Effectiveness of Psychobiotic Bifidobacterium breve BB05 in Managing Psychosomatic Diarrhea in College Students by Regulating Gut Microbiota: A Randomized, Double-Blind, Placebo-Controlled Trial
IF: 4.800 -
Activation of Piezo1 channels enhances spontaneous contractions of isolated human bladder strips via acetylcholine release from the mucosa
IF: 4.200 -
Neuroprotective Effect of Marrubium vulgare Extract in Scopolamine-Induced Cognitive Impairment in Rats: Behavioral and Biochemical Approaches
IF: 3.600Journal： Biology-Basel (2024)Reactivity：RatSample Type：frontal cortex,hippocampus tissue -
Dynamic changes in the hippocampal memory index and biochemical indices in Sprague Dawley rats exposed to intrauterine kola nut
IF: 3.200Journal： METABOLIC BRAIN DISEASE (2024)Reactivity：RatSample Type：hippocampal tissue -
Protective effects of berberine against diabetes-associated cognitive decline in mice
IF: 3.100Journal： ACTA DIABETOLOGICA (2024)Reactivity：MouseSample Type：brain tissue
实验操作视频
