ACH(Acetylcholine) ELISA Kit
中文名称：乙酰胆碱(ACH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用ACH抗原包被于酶标板上，实验时样品或标准品中的ACH与包被的ACH竞争生物素标记的抗ACH单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，ACH浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中ACH的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的ACH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Evaluation of possible neuroprotective effects of virgin coconut oil on aluminum-induced neurotoxicity in an in vitro Alzheimer's disease model
IF: 3.3 -
Persicaria minor ameliorates the cognitive function of chronic cerebral hypoperfusion rats: Metabolomic analysis and potential mechanisms
IF: 2.700 -
The effect of the calcium channel blocker nimodipine on hippocampal BDNF/Ach levels in rats with experimental cognitive impairment
IF: 1.900Journal： NEUROLOGICAL RESEARCH (2023)PMID：36598971Reactivity：RatSample Type：Hippocampal,prefrontal cortex -
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 1.600Journal： Journal of General and Family Medicine (2023)DOI：10.1002/jgf2.606PMID：37261039Reactivity：HumanSample Type：serum -
A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of the gut microbiota and relief from chronic constipation
IF: 6.317 -
Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 5.435 -
Guggulsterone Mediated JAK/STAT and PPAR-Gamma Modulation Prevents Neurobehavioral and Neurochemical Abnormalities in Propionic Acid-Induced Experimental Model of Autism
IF: 4.927 -
Survival and Neurogenesis-Promoting Effects of the Co-Overexpression of BCLXL and BDNF Genes on Wharton’s Jelly-Derived Mesenchymal Stem Cells
IF: 3.251 -
Nootropic effect of Syzygium polyanthum (Wight) Walp?leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia
Journal： Advances in Traditional Medicine (2022)Reactivity：RatSample Type：brain -
Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y
IF: 16.806
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
