ADMA(Asymmetrical Dimethylarginine) ELISA Kit
中文名称:不对称二甲基精氨酸(ADMA)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Universal
- 检测范围: 15.63-1000 ng/mL
- 灵敏度: 9.38 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用ADMA抗原包被于酶标板上,实验时样品或标准品中的ADMA与包被的ADMA竞争生物素标记的抗ADMA单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,ADMA浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中ADMA的浓度。 |
反应类型 | Competitive-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 ng/mL |
灵敏度 | 9.38 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的ADMA,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
Association between protein arginine N-methyltransferase 1 polymorphism and overt diabetic nephropathy: Role of asymmetric dimethylarginine in vascular tone
IF: 4.200Journal: Journal of Clinical and Translational Endocrinology (2024)Reactivity:HumanSample Type:SerumLevothyroxine therapy reduces endocan and total cholesterol concentrations in patients with subclinical hypothyroidism
IF: 3.800Blood pressure response to exercise in unaffected relatives of autosomal dominant polycystic kidney disease patients: an observational study
IF: 2.000Journal: INTERNATIONAL UROLOGY AND NEPHROLOGY (2023)PMID:36872421Reactivity:HumanSample Type:PlasmaRole of micro-RNAs 21, 124 and other novel biomarkers in distinguishing between group 1 WHO pulmonary hypertension and group 2, 3 WHO pulmonary hypertension
IF: 1.100Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.
IF: 5.058Early manifestation of aging-related vascular dysfunction in human penile vasculature—A potential explanation for the role of erectile dysfunction as a harbinger of systemic vascular disease
IF: 7.713Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature
IF: 6.313Cardiovascular Risk Factors and Their Relationship with Vascular Dysfunction in South African Children of African Ancestry
IF: 4.242Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity
IF: 2.288Journal: Obesity Research & Clinical Practice (2021)PMID:34147377Reactivity:HumanSample Type:plasmaGut permeability is associated with hypertension and measures of obesity but not with Endothelial Dysfunction in South African youth
IF: 0.927