AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]
![AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性： FCM
- 亚型： Mouse IgG2b, κ
- 宿主： Mouse
| Alternate Names | B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, B7-H, B7-H1, Programmed cell death ligand 1 |
| Clone No | |
| Isotype | Mouse IgG2b, κ |
| Leadtime |
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| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
| Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
|
| Background |
CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
|
| Immunogen | Recombinant Human PD-L1 protein |
| Abbre | PD-L1 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Applications | |
| Research Areas | Cancer Biomarkers;Costimulatory Molecules;Immunology |
| Concentration | ≥ 1 mg/mL |
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12个月 |
| Shipping |
Ice bag |
Human peripheral blood lymphocytes were activated for 3 days with PHA, then stained with 0.2 μg AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] (Right) and 0.2 μg Mouse IgG2b, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD3 PE-conjugated Monoclonal Antibody.
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Upregulation of Immune checkpoint PD-L1 in Colon cancer cell lines and activation of T cells by Leuconostoc mesenteroides
IF: 4.000Journal： WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2024)Reactivity：Human
| E-AB-F1133A | Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100 μg , 25 μg |
| E-AB-F1133B | Biotin Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100μg , 25μg |
| E-AB-F1133C | FITC Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F1133D | PE Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F1133E | APC Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F1133G | PE/Cyanine5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F1133H | PE/Cyanine7 Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
| E-AB-F1133J | PerCP/Cyanine5.5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] | 100Tests , 20Tests , 100Tests×2 |
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