AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2]
![AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性： FCM
- 亚型： Rat IgG2a, λ
- 宿主： Rat
| Alternate Names | SLAM family member, SLAMF, CD 150, CD150, CD150 antigen, Signaling lymphocytic activation molecule, SLAM family member 1, Slam, Slamf1, IPO-3 |
| Clone No | |
| Isotype | Rat IgG2a, λ |
| Leadtime |
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| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
|
| Background |
CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.
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| Immunogen | Recombinant Mouse CD150 protein |
| Abbre | CD150 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Applications | |
| Concentration | ≥ 1 mg/mL |
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12个月 |
| Shipping |
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C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2] (Right) and 0.2 μg Rat Ig2a, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.
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