AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]
![AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性： FCM
- 亚型： Armenian Hamster IgG
- 宿主： Armenian Hamster
| Alternate Names | CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E |
| Clone No | |
| Isotype | Armenian Hamster IgG |
| Leadtime |
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| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
|
| Background |
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
|
| Immunogen | Recombinant Mouse CD3ε protein |
| Abbre | CD3ε |
| Swissprot | |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Applications | |
| Research Areas | Immunology |
| Concentration | ≥ 1 mg/mL |
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12个月 |
| Shipping |
Ice bag |
C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] (Right) and 0.2 μg Armenian Hamster IgG, κ Isotype Control (Left), followed by Alexa Fluor® 647-conjugated Goat Anti- Armenian Hamster IgG Secondary Antibody, then anti-Mouse CD8a FITC-conjugated Monoclonal Antibody.
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